RSPO1 (Human) Recombinant Protein
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Specification
Product Description
Human RSPO1 (Q2MKA7) recombinant protein expressed in CHO cells.
Sequence
RISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA
Host
Mammals
Theoretical MW (kDa)
25.6
Form
Lyophilized
Purity
>= 95%
Endotoxin Level
<= 1 EUs/ug (Kinetic LAL) (50% confidence)
Activity
ED50 <= 50 ng/mL
Luciferase induction in HEK-293 STF cells in the presence of murine Wnt-3a
The values provided above are minimum expected values to pass internal requirements.Quality Control Testing
Reducing and Non-Reducing SDS PAGE
Conformation
Monomer
Storage Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing PBS.
Storage Instruction
Stored at -20°C to-80°C for 12 month.
After reconstitution with sterile water at 0.1 mg/mL, store at -20°C to -80°C for 3 months, store at 4°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
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Gene Info — RSPO1
Entrez GeneID
284654Protein Accession#
Q2MKA7Gene Name
RSPO1
Gene Alias
CRISTIN3, FLJ40906, RSPO
Gene Description
R-spondin homolog (Xenopus laevis)
Omim ID
609595Gene Ontology
HyperlinkGene Summary
This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq
Other Designations
OTTHUMP00000180612|OTTHUMP00000180613|R-spondin1|roof plate-specific spondin
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