SPHK2 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human SPHK2 (NP_001191089) full-length recombinant protein with N-terminal GST tag expressed in baculovirus infected Sf21 cells.
Theoretical MW (kDa)
92
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione Sepharose chromatography
Purity
95 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The hosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Sphingosine. ATP: 400 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — SPHK2
Entrez GeneID
56848Protein Accession#
NP_001191089.1Gene Name
SPHK2
Gene Alias
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Gene Description
sphingosine kinase 2
Omim ID
607092Gene Ontology
HyperlinkOther Designations
sphingosine kinase type 2 isoform
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Interactome
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Pathway
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