Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 8-10 ng/mL.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from solutions contain no sodiun azide nor carrier protein.
Store at -20°C. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.