Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by its ability to inhibit 5.0 ng/mL of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/mL of Nog.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from solutions contain no sodiun azide nor carrier protein.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Due to solubility reasons the protein should be kept at low pH. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.