Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by its ability to inhibit MC3T3/E1 osteoblasts cell differentiation.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 20 mM Tris (pH 8.8, 200 mM L-Arginine).
Store at -20°C. Reconstitute in 20 mM Tris, pH 8.8 and 150 mM NaCl to a concentration of 0.1-1.0 mg/mL. Slow to dissolve. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at-20°C to -80°C.