Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
The ED50 was determined by its ability to intensify the IL-6 induced growth inhibition of murine M1 cells is <= 5.0 ng/mL, in the presence of 20 ng/mL of rhIL-6.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 1x PBS pH 7.2.
Store at -20°C. Reconstitute in 1x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C
Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq