Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
The ED50 was determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is <= 5.0 ng/mL (in the presence of 0.5 ng/mL of IL-4), corresponding to a specific activity of >= 2 x 105 units/mg.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 1x PBS pH 7.0.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. [provided by RefSeq