Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1 ng/mL of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5 ug/mL.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 1x PBS.
Store at -20°C. Reconstitute in 1x PBS pH 7.2 to a concentration of 0.1- 1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. [provided by RefSeq