Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is <= 10.0 ng/mL, corresponding to a specific activity of >= 1 x 105 units/mg.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 0.5x PBS, pH 7.5.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). [provided by RefSeq