Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by the dose dependent activation of NF-kappaB in a RAW264 cell line based reporter system, using a CD14 concentration range of 20 ng/uL to 200 ng/uL. The NF-kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/uL to 1.0 ng/uL bacterial LPS.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 10mM Sodium Phosphate, pH 7.5.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq