Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
RSPO2 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 10 mM Sodium Phosphate (pH 7.5, 150 mM NaCl).
Store at -20°C. Reconstitute in water to a concentration of 0.1-0.5 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.