Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by its ability to chemoattract human fibroblasts using a concentration range of 1.0-10.0 ng/mL.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 10mM Sodium Phosphate, pH 7.5.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM