Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PLA2G7 concentration of 10.0 ug/mL, 50% cleavage was achieved at an incubation time of approximately 2 minutes.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 10mM Tris, pH 8.5.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
The PLA2G7 gene encodes platelet-activating factor (PAF) acetylhydrolase (EC 220.127.116.11), a secreted enzyme that catalyzes the degradation of PAF to inactive products by hydrolysis of the acetyl group at the sn-2 position, producing the biologically inactive products LYSO-PAF and acetate.[supplied by OMIM
OTTHUMP00000016555,phospholipase A2, group VII,platelet-activating factor acetylhydrolase