Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by its ability to neutralize the stimulation of U937 cells treated with 10 ng/mL of soluble RANKL (sRANKL).
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 10 mM Tris (pH 7.5, 75 mM NaCl).
Store at -20°C. Reconstitute in 5 mM Tris, pH 7.5 to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq