Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
Determined by an in vivo assay using healthy wild type male mice (C57BL/6J). Mice were treated via intraperitoneal injection once at a dose of 4 ug NUCB2/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 0.1% TFA.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.