Measured by its ability to induce fluorescence in Lin28 reporter cells (293 cells transfected with fluorescent protein genes under Lin28 control). Optimum activity was achieved at 20 ug/ml after incubation for 72 hr.
SDS-PAGE Activity assay The optimal working dilution should be determined by the end user.
Lyophilized from 10mM Sodium Citrate, pH 3.0.
Store at -20°C. Reconstitute in water to a concentration of 1.0 mg/mL. Do not vortex. For extended storage, it is recommended to be further diluted in buffer containing carrier protein (e.g. 0.1% BSA) and stored in aliquots at -20°C to -80°C.