Determined by the stimulation of proliferation with murine NIH-3T3 cells. Recombinant human Klotho is effective at the range of 0.5-2.0 ug/ml.
SDS-PAGE Activity assay The optimal working dilution should be determined by the end user.
Lyophilized from 15mM Sodium Phosphate pH 7.3.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to be further diluted in buffer containing carrier protein (e.g. 0.1% BSA) and stored in aliquots at -20°C to -80°C.
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq