The ED50 determined by the stimulation of the proliferation with murine MC-9 cells is < 10 ng/ml, corresponding to a specific activity of > 1.0 x 105 units/mg.
SDS-PAGE Activity assay The optimal working dilution should be determined by the end user.
Lyophilized from 5mM Sodium Citrate, pH 4.0.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to be further diluted in buffer containing carrier protein (e.g. 0.1% BSA) and stored in aliquots at -20°C to -80°C.