The bioactivity is determined by its chemoattractivity with human CD4+ T-Lymphocytes with a concentration range of 50.0-100.0 ng/ml.
SDS-PAGE Activity assay The optimal working dilution should be determined by the end user.
Lyophilized from 5mM Sodium Phosphate, pH 5.5 and 0.5mM DTT.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to be further diluted in buffer containing carrier protein (e.g. 0.1% BSA) and stored in aliquots at -20°C to -80°C.
The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq