Human IL13 (P35225) partial recombinant protein expressed in E. coli. This variant of IL-13, a mature 114 amino acid protein with a substitution of Q for R at position 112, shows enhanced functional activity compared with wild type IL-13.
This IL-13 analog, with a substitution of Q for R at position 112, measured by the in vitro dose dependent activation of STAT6 and IL-13 dependent gene induction in transfected A201.1 cells. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13, as measured by the induction of airway hyper-responsiveness.
SDS-PAGE Activity assay The optimal working dilution should be determined by the end user.
Lyophilized from 10mM HCl.
Store at -20°C. Reconstitute in water to a concentration of 0.5-1.0 mg/mL. Do not vortex. Note: Allow the reconstituted vial to sit at 4°C for more than 2 hours before use. For extended storage, it is recommended to be further diluted in buffer containing carrier protein (e.g. 0.1% BSA) and stored in aliquots at -20°C to -80°C.
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq