The ED50 was determined by the dose-dependent stimulation of the proliferation of murine T11 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
SDS-PAGE Activity assay The optimal working dilution should be determined by the end user.
Lyophilized from solutions contain no sodiun azide nor carrier protein.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to be further diluted in buffer containing carrier protein (e.g. 0.1% BSA) and stored in aliquots at -20°C to -80°C.