Determined by its ability to inhibit IGF-II induced proliferation of human MCF-7 cells. The expected ED50 for this effect is 0.1-0.4ug/ml.
SDS-PAGE Activity assay The optimal working dilution should be determined by the end user.
Lyophilized from 10mM Sodium Citrate, pH 3.5.
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to be further diluted in buffer containing carrier protein (e.g. 0.1% BSA) and stored in aliquots at -20°C to -80°C.