Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug).
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is <= 5.0 ng/ml, corresponding to a specific activity of >= 2 x 105 units/mg.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from 20 mM Tris (pH 9.0, 0.1 mM Calcium Chloride and 100 mM Arginine).
Store at -20°C. Reconstitute in water to a concentration of 0.5-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.