The ED50 was determined by the dosedependent proliferation of the RPMI 8226 cells. The expected ED50 for this effect is 15.0-20.0 ng/mL.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile 10 mM HCl
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL and keep it at low pH. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq