Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1.0 ug/mL.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile 10 mM Sodium Phosphate, pH 7.0
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq