Assay 1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/mL. Assay 2: TNFSF12 weakly induces the death of HT29 cells when cultured in the presence of IFNG. The ED50 for this effect is between 30-45 ng/mL.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile 10 mM Sodium Phosphate, pH 7.5 containing 0.1M L-Arginine
Store at -20°C. Reconstitute in 10 mM Sodium Phosphate, pH 7.5 to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Some transcripts that skip the last exon of this gene and continue with the second exon of the neighboring TNFSF13 gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq
APO3/DR3 ligand,TNF-related WEAK inducer of apoptosis