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TNFSF12 (Human) Recombinant ProteinBioActive

  • Catalog # : P6048
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  • Specification
  • Product Description:
  • Human TNFSF12 (O43508) partial recombinant protein expressed in Escherichia coli.
  • Sequence:
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 27.0
  • Reactivity:
  • Mouse
  • Form:
  • Lyophilized
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • 98%
  • Endotoxin Level:
  • <0.1 ng/ug (<1 EU/ug)
  • Activity:
  • Assay 1:
    The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/mL.
    Assay 2:
    TNFSF12 weakly induces the death of HT29 cells when cultured in the presence of IFNG. The ED50 for this effect is between 30-45 ng/mL.
  • Recommend Usage:
  • Activity assay
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from sterile 10 mM Sodium Phosphate, pH 7.5 containing 0.1M L-Arginine
  • Storage Instruction:
  • Store at -20°C.
    Reconstitute in 10 mM Sodium Phosphate, pH 7.5 to a concentration of 0.1-1.0 mg/mL. Do not vortex.
    For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.
  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 8742
  • Gene Name:
  • TNFSF12
  • Gene Alias:
  • APO3L,DR3LG,MGC129581,MGC20669,TWEAK
  • Gene Description:
  • tumor necrosis factor (ligand) superfamily, member 12
  • Gene Summary:
  • The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Some transcripts that skip the last exon of this gene and continue with the second exon of the neighboring TNFSF13 gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq
  • Other Designations:
  • APO3/DR3 ligand,TNF-related WEAK inducer of apoptosis
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