Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is <= 2.0 ug/mL of TWSG1.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile 10 mM Sodium Acetate, pH 6.0
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.