Determined by the cytolysis of mouse L929 cells in the presence of Actinomyocin D. The expected ED50 is <= 0.05 ng/mL, corresponding to a specific activity of >= 2 x 107 units/mg.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile 0.5x PBS, pH 7.1
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.