Determined by the cytolysis of mouse L929 cells in the presence of Actinomyocin D. The expected ED50 is <= 0.1 ng/mL, corresponding to a specific activity of >= 1 x 107 units/mg.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile 10 mM Sodium Phosphate, pH 7.5
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.