Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is <= 0.2 ng/mL, corresponding to a specific activity of >= 5 x 106 units/mg.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile solution with no additives
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.