Bovine, Chicken, Dog, Frog, Monkey, Mouse, Pig, Rabbit, Rat
Mammalian cell (HEK293) expression system
<0.1 ng/ug (<1 EU/ug)
Determined by TGFB1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is <= 0.05 ng/mL, corresponding to a specific activity of >= 2 x 107 units/mg.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile 0.1% TFA
Store at -20°C. Reconstitute in 10mM Citric Acid, pH 3.0 to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.
TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA (MIM 190170) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2 (MIM 190220), and TGFB3 (MIM 190230) all function through the same receptor signaling systems.[supplied by OMIM