Assay 1: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ug/mL. Assay 2: Determined by its ability to chemoattract human MCF-7 cells using a concentration range of 1.0-10.0 ng/mL.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from sterile solution with no additives
Store at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, to further dilute in buffer containing carrier protein (e.g. 0.1% BSA) and store in aliquots at -20°C to -80°C is recommended.
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq
spasmolysin,spasmolytic polypeptide,spasmolytic protein 1