Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per ug of enzyme.
Activity assay SDS-PAGE The optimal working dilution should be determined by the end user.
Lyophilized from solutions contain no sodiun azide nor carrier protein
Store at -20°C. Aliquot to avoid repeated freezing and thawing.