GARS (Human) recombinant protein
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Loading 2 ug protein in SDS-PAGE: Lane1: BSA, Lane2: His-GARS.
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Specification
Product Description
Human GARS full-length recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
79
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Multi-step chromatography.
Concentration
0.40 mg/ml
Purity
95.1%
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Loading 2 ug protein in SDS-PAGE: Lane1: BSA, Lane2: His-GARS.
Recommend Usage
SDS-PAGE
Western blot
ELISA
Antibody production
Protein Array
Activity assay
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 ( 20 ug/ml Kanamycin)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
Functional Study
SDS-PAGE
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Gene Info — GARS
Entrez GeneID
2617Gene Name
GARS
Gene Alias
CMT2D, DSMAV, GlyRS, HMN5, SMAD1
Gene Description
glycyl-tRNA synthetase
Gene Ontology
HyperlinkGene Summary
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq
Other Designations
Charcot-Marie-Tooth neuropathy 2D|Charcot-Marie-Tooth neuropathy, neuronal type, D|glycine tRNA ligase
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Interactome
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Pathway
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Disease
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