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PRMT3 (Human) Recombinant Protein

  • Catalog # : P5927
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  • Specification
  • Product Description:
  • Human PRMT3 (NP_005779, 2 a.a. - 531 a.a.) full-length recombinant protein with N-terminal GST tag expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 86.6
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • ≥90%
  • Activity:
  • Activity was determined using a radiometric assay with [3H]-S-adenosylmethionine incubated with core histones or the glycine arginine rich (GAR) region from fibrillarin at 30°C for 30 minutes or overnight at room temperature in buffer containing 50 mM Tris, pH 8.0, 150 mM sodium chloride, 3 mM MgCl2, and 5% glycerol.
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 8.0 (20% glycerol)
  • Storage Instruction:
  • Store at -80°C for 6 months.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • Autoradiograph of glycine arginine rich (GAR) peptide or core histones separated on SDS-PAGE following reaction with [3H]-SAM and PRMT3.
    Lane 1: PRMT3 (3 uM) with GAR peptide, Lane 2: PRMT3 (3 uM) with GAR peptide (Overnight reaction), Lane 3: PRMT3 (3 uM) with Core Histones and Lane 4: PRMT3 (3 uM) with Core Histones (Overnight reaction)


  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of PRMT3 (Human) Recombinant Protein (Cat # P5927) using protein specific antibody.
    Lane 1: 2.5 ug and lane 2: 5 ug.
  • Functional Study
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Functional Study
  • Gene Information
  • Gene Name:
  • PRMT3
  • Gene Alias:
  • HRMT1L3
  • Gene Description:
  • protein arginine methyltransferase 3
  • Gene Summary:
  • Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM
  • Other Designations:
  • HMT1 hnRNP methyltransferase-like 3,heterogeneous nuclear ribonucleoprotein methyltransferase-like 3,protein arginine N-methyltransferase 3
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