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GH1 (Human) Recombinant ProteinBioActive

  • Catalog # : P5926
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  • Specification
  • Product Description:
  • Human GH1 (205 a.a.) full-length recombinant protein with N-terminal His tag expressed in Nicotiana benthamiana.
  • Sequence:
  • Host:
  • Plants
  • Theoretical MW (kDa):
  • 22.9
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Non-transgenic plants (Nicotiana benthamiana) expression system
  • Purification:
  • Sequential chromatography (FPLC)
  • Purity:
  • > 97% by SDS-PAGE
  • Endotoxin Level:
  • < 0.04 EU/ug protein
  • Activity:
  • The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells and kerotinocytes migration using HaCaT cells.
  • Quality Control Testing:
  • 15% SDS-polyacrylamide gel and stained with Coomassie blue

    QC Testing of P5926
    Lane 1: 0.05 ug and lane 2: 0.3 ug
  • Storage Buffer:
  • Lyophilized from 10mM Phosphate Potasium buffer pH 7,6 and 0.05M NaCl
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • ED50 ≤ 0.04-0.1 ng/mL

  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of GH1 (Human) Recombinant Protein (Cat # P5926) using protein specific antibody.
    Lane 1: 0.05 ug and lane 2: 0.3 ug of GH1 (Human) Recombinant Protein
  • Functional Study
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 2688
  • Gene Name:
  • GH1
  • Gene Alias:
  • Gene Description:
  • growth hormone 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq
  • Other Designations:
  • pituitary growth hormone
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