Non-transgenic plants (Nicotiana benthamiana) expression system
Sequential chromatography (FPLC)
> 97% by SDS-PAGE
< 0.04 EU/ug protein
The specific activity was determined by a quantitative gene reported bioassay using human Type I interferon-sensitive. The activity is < 8 x 108 units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
Quality Control Testing:
0.3 ug/lane in 15% SDS-polyacrylamide gel and stained with Coomassie blue
Lyophilized from Tris HCl 0.05M buffer at pH 7.4 and 0.01% SDS
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.