Product Browser

Last updated: 2017/10/15

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

TNFSF11 (Human) Recombinant Protein

  • Catalog # : P5912
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Human TNFSF11 (O14788, 70 a.a. - 244 a.a.) partial recombinant protein with N-terminal His tag expressed in Nicotiana benthamiana.
  • Sequence:
  • HHHHHHHHHHAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
  • Host:
  • Plants
  • Theoretical MW (kDa):
  • 21-23
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Non-transgenic plants (Nicotiana benthamiana) expression system
  • Purification:
  • Sequential chromatography (FPLC)
  • Purity:
  • > 97% by SDS-PAGE
  • Endotoxin Level:
  • < 0.04 EU/ug protein
  • Quality Control Testing:
  • 1 ug/lane in 15% SDS-polyacrylamide gel and stained with Coomassie blue

    QC Testing of P5912
  • Storage Buffer:
  • Lyophilized from 10 mM PBS buffer pH 7.6 and 0.2 M NaCl
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of TNFSF11 (Human) Recombinant Protein (Cat # P5912) using protein specific antibody.
    Lane 1: 1 ug, lane 2: 0.5 ug and lane 3: 0.1 ug of TNFSF11 (Human) Recombinant Protein
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 8600
  • Gene Name:
  • TNFSF11
  • Gene Alias:
  • CD254,ODF,OPGL,OPTB2,RANKL,TRANCE,hRANKL2,sOdf
  • Gene Description:
  • tumor necrosis factor (ligand) superfamily, member 11
  • Gene Summary:
  • This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000178585,TNF-related activation-induced cytokine,osteoclast differentiation factor,osteoprotegerin ligand,receptor activator of nuclear factor kappa B ligand,tumor necrosis factor ligand superfamily, member 11
  • RSS
  • YouTube
  • Linkedin
  • Facebook