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CSF2 (Human) Recombinant ProteinBioActive

  • Catalog # : P5911
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Human CSF2 (P04141, 18 a.a. - 144 a.a.) full-length recombinant protein with N-terminal His tag expressed in Nicotiana benthamiana.
  • Sequence:
  • HHHHHHHHHHAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
  • Host:
  • Plants
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Non-transgenic plants (Nicotiana benthamiana) expression system
  • Purification:
  • Sequential chromatography (FPLC)
  • Purity:
  • > 97% by SDS-PAGE
  • Endotoxin Level:
  • < 0.04 EU/ug protein
  • Activity:
  • The activity of recombinant human GM-CSF is determined by the dose-dependent induction of human TF-1 proliferation cell.
  • Quality Control Testing:
  • 500 ng/lane in 15% SDS-polyacrylamide gel and stained with Coomassie blue

    QC Testing of P5911
    It migrates as a broad band between 15 and 25 kDa due to post-translation modification, in particular glycosilation.
  • Storage Buffer:
  • Lyophilized from 10 mM PBS buffer pH 7.6 and 0.2 M NaCl
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water to a concentration of 25-50 ng/ul, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • Cell proliferation was measured by MTT method. ED50 is ≤ 0.05 ng/mL


  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of CSF2 (Human) Recombinant Protein (Cat # P5911) using protein specific antibody.
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 1437
  • Gene Name:
  • CSF2
  • Gene Alias:
  • GMCSF,MGC131935,MGC138897
  • Gene Description:
  • colony stimulating factor 2 (granulocyte-macrophage)
  • Gene Summary:
  • The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq
  • Other Designations:
  • colony stimulating factor 2,granulocyte-macrophage colony stimulating factor,molgramostin,sargramostim
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