Human GDNF (150 a.a.) full-lenth recombinant protein with His tag expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
Host:
Plants
Theoretical MW (kDa):
25
Reactivity:
Human
Form:
Lyophilized
Preparation Method:
Hordeum vulgare (barley) expression system
Purification:
Chromatography
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kineticassay.
Activity:
Activity has been measured by cell-based proliferation assay using rat C6 cells. ED50 is 1.96 ?g/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Storage Buffer:
Lyophilized from PBS, pH 7.2
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq