FGF7 (Human) Recombinant Protein
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of FGF7 (Human) Recombinant Protein (Cat # P5880) using protein specific antibody.
Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human FGF7 (180 a.a.) full-lenth recombinant protein with His tag expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
Host
Plants
Theoretical MW (kDa)
14
Reactivity
Human
Form
Lyophilized
Preparation Method
Hordeum vulgare (barley) expression system
Purification
Chromatography
Purity
> 95% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kineticassay.
Activity
Activity has been measured by cell-based proliferation assay using 4MBr5 rhesus monkey epithelial cells. ED50 is typically below 4 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of FGF7 (Human) Recombinant Protein (Cat # P5880) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — FGF7
Entrez GeneID
2252Gene Name
FGF7
Gene Alias
HBGF-7, KGF
Gene Description
fibroblast growth factor 7 (keratinocyte growth factor)
Omim ID
148180Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq
Other Designations
fibroblast growth factor 7|heparin-binding growth factor 7|keratinocyte growth factor
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