Product Browser

Last updated: 2017/10/22
  • Related Product Showcase
  • By Research Area

  • By Antibody/Protein

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

TNF (Human) Recombinant ProteinBioActive

  • Catalog # : P5879
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Human TNF (174 a.a.) full-lenth recombinant protein with His tag expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
  • Host:
  • Plants
  • Theoretical MW (kDa):
  • 24
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Preparation Method:
  • Hordeum vulgare (barley) expression system
  • Purification:
  • Chromatography
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kineticassay.
  • Activity:
  • Activity has been measured by cell-based cytotoxicity assay using 929 cells. ED50 is 0.4 ng/mL.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5879
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.2
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of TNF (Human) Recombinant Protein (Cat # P5879) using protein specific antibody.
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 7124
  • Gene Name:
  • TNF
  • Gene Alias:
  • DIF,TNF-alpha,TNFA,TNFSF2
  • Gene Description:
  • tumor necrosis factor (TNF superfamily, member 2)
  • Gene Summary:
  • This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
  • Other Designations:
  • APC1 protein,OTTHUMP00000029281,OTTHUMP00000037669,TNF superfamily, member 2,TNF, macrophage-derived,TNF, monocyte-derived,cachectin,tumor necrosis factor alpha
  • Related Disease
  • RSS
  • YouTube
  • Linkedin
  • Facebook