Mouse Vegfa (164 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Theoretical MW (kDa):
Barley grain (Hordeum vulgare) expression system
> 98% by SDS-PAGE
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Bioactivity is assayed by measuring the dose dependent effect of mouse VecgfaVEGF164 on proliferation of Human Umbilical Vein Endothelial Cells (HUVEC) cells. This growth factor was tested by an independent company and exhibits an ED50 of 2.9 ng/mL corresponding to 0.34x106 U/mg specific activity, a value comparable to their in-house standard measured in parallel.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Lyophilized from PBS pH 7.2
Store at -20°C on dry atmosphere. After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.