Human FGF2 (146 a.a.) partial recombinant protein with ERHV-His tag expressed in Barley grain (Hordeum vulgare).
Theoretical MW (kDa):
Barley grain (Hordeum vulgare) expression system
> 95% by SDS-PAGE
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Bioactivity of recombinant human FGFB is assayed by measuring its dose dependent effect on proliferation of 3T3 cells. The ED50 for this effect using FGF basic 146 is typically < 0.4 ng/mL, corresponding to a specific activity > 2.5 x106 U/mg.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Lyophilized from PBS pH 7.2
Store at -20°C on dry atmosphere. After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq