Human IL4 (130 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Theoretical MW (kDa):
Barley grain (Hordeum vulgare) expression system
> 95% by SDS-PAGE
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
The bioactivity is determined by the cell proliferation assay for IL4 for dose-dependent cell proliferation of human TF-1 cells. The ED50 value for this effect is typically < 0.3 ng/mL, corresponding to a specific activity > 3.3 x 10e6 units/mg. Optimal concentration should be determined for specific applications and cell lines.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Lyophilized from PBS, pH7,2.
Store at -20°C on dry atmosphere. After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq