Product Browser

Last updated: 2016/12/4
  • Related Product Showcase
  • By Research Area

  • By Antibody/Protein

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

IL4 (Human) Recombinant ProteinBioActive

  • Catalog # : P5823
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Human IL4 (130 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
  • Host:
  • Plants
  • Theoretical MW (kDa):
  • 17.2
  • Form:
  • Lyophilized
  • Preparation Method:
  • Barley grain (Hordeum vulgare) expression system
  • Purification:
  • Chromatography
  • Concentration:
  • 100 ug/mL
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
  • Activity:
  • The bioactivity is determined by the cell proliferation assay for IL4 for dose-dependent cell proliferation of human TF-1 cells. The ED50 value for this effect is typically < 0.3 ng/mL, corresponding to a specific activity > 3.3 x 10e6 units/mg. Optimal concentration should be determined for specific applications and cell lines.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5823
  • Storage Buffer:
  • Lyophilized from PBS, pH7,2.
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of IL4 (Human) Recombinant Protein (Cat # P5823) using protein specific antibody.
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 3565
  • Gene Name:
  • IL4
  • Gene Alias:
  • BCGF-1,BCGF1,BSF1,IL-4,MGC79402
  • Gene Description:
  • interleukin 4
  • Gene Summary:
  • The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • B cell growth factor 1,B_cell stimulatory factor 1,lymphocyte stimulatory factor 1
  • Related Disease
  • RSS
  • YouTube
  • Linkedin
  • Facebook