IL2 (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Human IL2 (133 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Host
Plants
Theoretical MW (kDa)
22
Form
Lyophilized
Preparation Method
Barley grain (Hordeum vulgare) expression system
Purification
Chromatography
Concentration
100 ug/mL
Purity
> 98% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Activity
The bioactivity of recombinant human IL2 is determined by measuring the dose dependent effect on the proliferation of CTLL-2 cells. The ED50 for this effect is typically < 0.25 ng/mL, which corresponds to a specific activity > 4 x106 U/mg.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from PBS, pH 7.2
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
-
Applications
Western Blot (Recombinant protein)
Western blot analysis of IL2 (Human) Recombinant Protein (Cat # P5822) using protein specific antibody.Functional Study
SDS-PAGE
-
Gene Info — IL2
Entrez GeneID
3558Protein Accession#
A14844Gene Name
IL2
Gene Alias
IL-2, TCGF, lymphokine
Gene Description
interleukin 2
Omim ID
147680Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq
Other Designations
T cell growth factor|aldesleukin|interleukin-2|involved in regulation of T-cell clonal expansion
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Coupling programmed cell death 1-positive tumor-infiltrating T cells with anti-programmed cell death 1 antibody improves the efficacy of adoptive T-cell therapy.
Jiacheng Chu, Chenya Wang, Qingle Ma, Huaxing Dai, Jialu Xu, Edikan A Ogunnaike, Fei Peng, Xiaolin Shi, Chao Wang.
Cytotherapy 2022 Mar; 24(3):291.
Application:Func, Mouse, Mouse dendritic cells, Mouse splenocytes, Mouse tumor-infiltrating lymphocytes.
-
Coupling programmed cell death 1-positive tumor-infiltrating T cells with anti-programmed cell death 1 antibody improves the efficacy of adoptive T-cell therapy.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com