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Last updated: 2019/12/15
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IL2 (Human) Recombinant Protein BioActive

  • Catalog # : P5822
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  • Specification
  • Product Description:
  • Human IL2 (133 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
  • Host:
  • Plants
  • Theoretical MW (kDa):
  • 22
  • Form:
  • Lyophilized
  • Preparation Method:
  • Barley grain (Hordeum vulgare) expression system
  • Purification:
  • Chromatography
  • Concentration:
  • 100 ug/mL
  • Purity:
  • > 98% by SDS-PAGE
  • Endotoxin Level:
  • Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
  • Activity:
  • Bioactivity of recombinant human IL2 is assayed by measuring its dose dependent effect on proliferation of CTLL-2 cells. The ED50 for this effect is typically < 0.25 ng/mL, corresponding to a specific activity > 4 x106 U/mg.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5822
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.2
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of IL2 (Human) Recombinant Protein (Cat # P5822) using protein specific antibody.
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 3558
  • Gene Name:
  • IL2
  • Gene Alias:
  • IL-2,TCGF,lymphokine
  • Gene Description:
  • interleukin 2
  • Gene Summary:
  • The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq
  • Other Designations:
  • T cell growth factor,aldesleukin,interleukin-2,involved in regulation of T-cell clonal expansion
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