Human IL2 (133 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Host:
Plants
Theoretical MW (kDa):
22
Form:
Lyophilized
Preparation Method:
Barley grain (Hordeum vulgare) expression system
Purification:
Chromatography
Concentration:
100 ug/mL
Purity:
> 98% by SDS-PAGE
Endotoxin Level:
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Activity:
Bioactivity of recombinant human IL2 is assayed by measuring its dose dependent effect on proliferation of CTLL-2 cells. The ED50 for this effect is typically < 0.25 ng/mL, corresponding to a specific activity > 4 x106 U/mg.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Storage Buffer:
Lyophilized from PBS, pH 7.2
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq
Other Designations:
T cell growth factor,aldesleukin,interleukin-2,involved in regulation of T-cell clonal expansion