Human BMP2 (114 a.a.) full-length recombinant protein expressed in Nicotiana tabacum.
Theoretical MW (kDa):
Nicotiana tabacum expression system
> 95% by SDS-PAGE
Endotoxin level is less than 0.05 EU/ug as measured by LAL method.
The bioactivity of recombinant human BMP2 was determined by its dose dependent induction of alkaline phosphatase production by ATDC-5 mouse chondrogenic cells. The ED50 value for this batch of BMP-2 was determined to be 41 ng/mL, corresponding to 2.4 x 104 U/mg specific activity.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Lyophilized from sterile solution with 20 mM MES, pH 5
Store at -20°C on dry atmosphere. After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq