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Last updated: 2016/12/4
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Lif (Mouse) Recombinant ProteinBioActive

  • Catalog # : P5813
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  • Specification
  • Product Description:
  • Mouse Lif (188 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
  • Host:
  • Plants
  • Theoretical MW (kDa):
  • 28-30
  • Form:
  • Lyophilized
  • Preparation Method:
  • Barley grain (Hordeum vulgare) expression system
  • Purification:
  • Chromatography
  • Concentration:
  • 100 ug/mL
  • Purity:
  • > 98% by SDS-PAGE
  • Endotoxin Level:
  • Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
  • Activity:
  • The bioactivity was determined by its ability to induce differentiation of murine M1 myeloid leukemia cells. The ED50 value for this effect is typically < 1 ng/mL, corresponding to a specific activity of > 5 x 107 U/mg (where 50 U is defined as the amount of mouse Lif required to induce differentiation of 50% of M1 cells).
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P5813
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.2
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of Lif (Mouse) Recombinant Protein (Cat # P5813) using protein specific antibody.
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • Lif
  • Gene Alias:
  • -
  • Gene Description:
  • leukemia inhibitory factor
  • Other Designations:
  • OTTMUSP00000005253
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