Mouse Lif (188 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Theoretical MW (kDa):
Barley grain (Hordeum vulgare) expression system
> 98% by SDS-PAGE
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
The bioactivity was determined by its ability to induce differentiation of murine M1 myeloid leukemia cells. The ED50 value for this effect is typically < 1 ng/mL, corresponding to a specific activity of > 5 x 107 U/mg (where 50 U is defined as the amount of mouse Lif required to induce differentiation of 50% of M1 cells).
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Lyophilized from PBS, pH 7.2
Store at -20°C on dry atmosphere. After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.