TYRO3 (Human) Recombinant Protein
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Specification
Product Description
Human TYRO3 (NP_006284.2, 453 a.a. - 890 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
76
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
82 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip3000.
Substrate : CSKtide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TYRO3
Entrez GeneID
7301Protein Accession#
NP_006284.2Gene Name
TYRO3
Gene Alias
BYK, Brt, Dtk, FLJ16467, RSE, Sky, Tif
Gene Description
TYRO3 protein tyrosine kinase
Omim ID
600341Gene Ontology
HyperlinkOther Designations
Tyro3 protein tyrosine kinase (sea-related receptor tyrosine kinase)|tyrosine-protein kinase receptor TYRO3
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Interactome
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Disease
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