TTK (Human) Recombinant Protein
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Specification
Product Description
Human TTK (NP_003309.2, 1 a.a. - 857 a.a.) full-length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
123
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
85 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with biotinylated peptide in streptavidin-coated ELISA plate. Phosphorylation was detected by HRP-labeled anti-phospho antibody.Substrate : Lyn substrate peptide. ATP: 20 µM.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TTK
Entrez GeneID
7272Protein Accession#
NP_003309.2Gene Name
TTK
Gene Alias
ESK, FLJ38280, MPS1, MPS1L1, PYT
Gene Description
TTK protein kinase
Omim ID
604092Gene Ontology
HyperlinkOther Designations
phosphotyrosine picked threonine kinase (PYT)
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Interactome
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Pathway
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Disease
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